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. 2010 Jan 20;30(3):1073–1085. doi: 10.1523/JNEUROSCI.3145-09.2010

Figure 9.

Figure 9.

SNL-induced upregulation of pCREB. Reversal after intrathecal anti-CaV1.2 TP10-PNA application. A, Radioactive immunolabeling of pCREB in the spinal dorsal horn (transverse section in the lumbar spinal cord). B, Quantification in superficial dorsal horn. The values represent the difference in surfacic activity between ipsilateral and contralateral sides expressed in percentage of the contralateral side (ΔSA = [(SAi − SAc)/SAc] × 100; see Materials and Methods). C, Quantification in deep dorsal horn. In SNL animals (A, left), pCREB expression increased (arrowhead) ipsilateral to the ligature (asterisk), both in superficial (black bar in B) and deep (black bar in C) laminae. Intrathecal application of anti-CaV1.2 TP10-PNA (A, right) suppressed the ipsilateral increase in pCREB, both in the superficial (arrowhead in A, right, white bar in B) and deep laminae (arrow in A, right, white bar in C). In contrast, CaV1.3 knockdown did not modify the SNL-induced increase in pCREB expression, either in the superficial (gray bar in B) or in the deep (gray bar in C) laminae. One star, p < 0.05; two stars, p < 0.01.