Table 2.

LDCV from CgB-KO chromaffin cells cannot uptake more catecholamines

	Imax (pA)	Q (pC)	t1/2 (ms)	n
WT	20.7 ± 4.4	0.31 ± 0.1	11.1 ± 0.9	10
WT, l-DOPA	51.7 ± 7.6†	0.95 ± 0.2¥	16.1 ± 1.7§	10
CgB-KO	35.3 ± 3.2	0.43 ± 0.03	7.9 ± 0.6	11
CgB-KO, l-DOPA	36.2 ± 2.3	0.33 ± 0.04	6.8 ± 0.9	10

Cells from WT or CgB-KO mice were incubated with culture media or 100 μm l-DOPA for 90 min. Exocytosis was monitored by single cell amperometry. I_max, Spike maximal height; Q, net CA content; and t_1/2, the spike duration at half-height. ^†p = 0.007; ^¥p = 0.011; ^§p = 0.035. Values of p were obtained with the Mann–Whitney test. Note that the experiments done with WT and CgB-KO cells were conducted in different days using different electrodes; for this reason quantitative analysis between these two groups cannot be performed.