Table 3.
Proteins present in LDCV of chromaffin cells identified by MALDI-TOF MS
| ID-Spot | Protein identity | NCBI No (gi) | MW (Da) | pI | Matchedpeptide | Sequencecoverage (%) | Mascotscore | Missedcleavage | Fold change |
|---|---|---|---|---|---|---|---|---|---|
| 1 | Chromogranin-B | 50409 | 77,895 | 5.01 | 14 | 22 | 122 | 3 | Only WT |
| 2 | Secretogranin II, precursor | 417771 | 70,600 | 4.69 | 18 | 31 | 194 | 2 | * |
| 3 | Chromogranin-A | 20071660 | 51,929 | 4.65 | 15 | 27 | 114 | 4 | * |
| 4 | Aldehyde dehydrogenase 2, mitochondrial | 26330458 | 57,015 | 7.53 | 11 | 25 | 146 | 1 | +2.4 |
| 5 | Albumin | 26341396 | 67,013 | 5.49 | 11 | 21 | 94 | 1 | −2.3 |
| 6 | Heat shock protein 1 (Chaperonin) | 26353954 | 61,089 | 5.67 | 9 | 20 | 87 | 1 | +1.8 |
| 7 | Protein disulfide isomerase-associated 3 | 26353794 | 57,103 | 5.78 | 9 | 23 | 106 | 2 | +1.5 |
| 8 | Vimentin | 2078001 | 51,590 | 4.96 | 8 | 31 | 79 | 1 | Only KO |
| 9 | Fibrinogen, γ polypeptide | 18043449 | 50,044 | 5.54 | 18 | 37 | 137 | 2 | Only KO |
| 10 | Fibrinogen, β polypeptide | 21619364 | 55,402 | 6.68 | 10 | 23 | 100 | 1 | Only KO |
| 11 | Fibrinogen, α polypeptide | 13529485 | 61,801 | 7.16 | 23 | 33 | 175 | 2 | Only KO |
| 12 | ATP synthase, H+ transporting mitochondrial F1 complex, β subunit | 28302366 | 56,265 | 5.19 | 19 | 55 | 230 | 1 | +1.3 |
| 13 | Acyl-coenzyme A dehydrogenase, short chain | 192659 | 45,203 | 8.96 | 9 | 28 | 74 | 2 | Only KO |
| 14 | Apolipoprotein E | 54035417 | 35,901 | 5.56 | 12 | 28 | 91 | 2 | +2.6 |
| 15 | Prohibitin | 12832901 | 29,859 | 5.57 | 9 | 55 | 132 | 1 | +1.5 |
| 16 | Chapsin 110/ Discs large homolog 2 (Drosophila) | 26338173 | 54,421 | 6.93 | 6 | 18 | 68 | 1 | −2.1 |
*Not quantified, multiple postprocessing proteins.