Figure 3.

nNOS is required for the behavioral effects of glucocorticoids. A, B, Immobility time in the TST and FST (A, n = 11) and sucrose preference (B, n = 10–11) of the adult mice treated with corticosterone alone (40 mg/kg/d s.c.) or in combination with 7-NI (30 mg/kg/d i.p.) for 21 d. C, D, Immobility time in the TST and FST (C) and sucrose preference (D) of the adult mice treated with corticosterone alone (40 mg/kg s.c.) or in combination with 10 μm 7-NI (hippocampal microinjection, 2 μl; n = 10–11). Corticosterone was administrated for 21 d and started at day 4 after 7-NI microinjection. E–G, Immobility time in the TST and FST (E), sucrose preference (F), and body weight gain (G) of the adult mice exposed to CMS for 21 d with or without 10 μm 7-NI (hippocampal microinjection, 2 μl; n = 11–12). CMS was started at day 4 after hippocampal 7-NI microinjection. H, I, Immobility time in the TST (H) and FST (I) of the nNOS KO (n = 9–10) and WT (n = 14) mice treated with 10 μm corticosterone (hippocampal microinjection, 2 μl). The TST and FST were performed at day 21 after hippocampal microinjection. Error bars denote SEM; *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA (A–G), two-way ANOVA (H–I). Cort, Corticosterone.