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. 2011 May 25;31(21):7920–7926. doi: 10.1523/JNEUROSCI.2460-10.2011

Figure 2.

Figure 2.

Ablation of Robo-2 expression in OSN axons. A–P, Coronal sections of OBs from E13, E14, and P0 robo-2+/lox; OMP-Cre (A–D, I–L) or robo-2lox/lox; OMP-Cre (E–H, M–P) embryos stained with Robo-2 (A, B, D, E, F, H, I, K, L, M, O, P), OMP (C, D, G, H), or β-III tubulin antibodies (J, K, N, O). The Robo-2 expression observed on OSN axons (arrowheads) in robo-2+/lox; OMP-Cre mice (A, B, I) is diminished at E13 (E) and ablated at E14 (F) and P0 (M) in robo-2lox/lox; OMP-Cre mice. In contrast, Robo-2 is still expressed in the mitral cell layer of the OB in robo-2lox/lox; OMP-Cre mice (P). MCL, Mitral cell layer; ONL, olfactory nerve layer. Scale bars: A–H, 125 μm; I–P, 50 μm. Q–S, Coronal sections of OE from E13 MOR1–3-tau-lacZ embryos stained with β-gal (Q, S) and OMP (R, S) antibodies. While the majority of MOR1–3-expressing cells also express OMP (arrowheads) at that stage of development, some MOR1–3-positive cells do not express OMP (asterisk). The late onset of OMP expression in a portion of dorsal OSNs may explain the residual expression of Robo-2 observed on OSN axons at E13 in robo-2lox/lox; OMP-Cre mice. Scale bar, 25 μm.