Figure 2.

coiled mutants show similar BBB defects as neurexinIV mutants. Differently sized fluorescent-labeled dextran molecules were injected into stage 17 embryos of the following genotypes: w¹¹¹⁸; coiled^f05607; nrxIV⁴³⁰⁴; and moody^Δ17. Images shown were taken 2 min after injection. The different sized dextrans are indicated; all images were taken with a Carl Zeiss LSM 710 microscope. Homozygous embryos were recognized using GFP-labeled balancer chromosomes. A–C, A w¹¹¹⁸ embryo is used as wild-type control. D–F, In coiled mutants, smaller dyes readily penetrated into the nerve cord. Larger dyes penetrate into the nerve cord but not as easily as the 10 kDa dextran. G–I, In neurexinIV (nrxIV) mutant embryos, all sizes of dextran molecules penetrated comparable with coiled mutants. J–L, Dye penetration in moody mutants. M, Quantification of 10 kDa dextran uptake over 60 min in the genotypes indicated.