Figure 8.

Symmetric Coiled expression is required for septate junction formation. A, Frontal view of a single confocal section of a stage 16 mutant coiled embryo expressing Coiled and GFP in the engrailed pattern, stained for Coracle (red) and GFP (green) expression. The plain of view is indicated in the orthogonal section (arrow). The length of septate junctions is rescued to the normal length among cells within the engrailed domain (hexagon), whereas between mutant and rescued cells the Coracle expression domain is expanded as in the mutant tissue (oval and rectangle, respectively). The relative Coracle expression domain length is quantified in B. n = 60 cells. +/+ indicates Coracle expression domain length between rescued cells, +/− indicates Coracle expression domain length between rescued and mutant cells, and −/− indicates Coracle expression domain length between two mutant cells.