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. 2011 Sep 28;31(39):13972–13980. doi: 10.1523/JNEUROSCI.1289-11.2011

Figure 6.

Figure 6.

EHD modulates dynamin function in vitro. A, Vesiculation from rhodamine-labeled SUPER templates induced by application of dynamin in the constant presence of GTP (1 mm GTP and ATP present in A and B). The trace with the response to dynamin (dyn) is superimposed on the trace preceding addition of dynamin (-protein). B, Vesiculation was suppressed when dynamin was coapplied with l-EHD. C, Reduced inhibitory effect of l-EHD on dynamin-induced vesiculation in the constant presence of GTP after replacement of ATP with ATPγS (1 mm). D, Application of dynamin in the constant presence of GTPγS induced formation of narrow tubules (1 mm GTPγS and ATP present in D–F). E, Tubule formation was suppressed when dynamin was coapplied with l-EHD. F, Reduced inhibitory effect of l-EHD on dynamin-induced tubulation in the constant presence of GTPγS after replacement of ATP with ATPγS. Scale bars, 5 μm.