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. 2019 Aug;25(8):935–947. doi: 10.1261/rna.071191.119

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© 2019 Wang et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 6. — Circular dichroism spectroscopy indicates that C9 40-mer RNA lacks the hallmarks of G-Q structures and has a CD spectrum matching that of a designed hairpin structure. (A–C) C9 40-mer RNA. (D–F) TERRA 24-mer RNA. (G–I) Hairpin no-G-Q 40-mer RNA. The first column of spectra shows the cation dependence; 100 mM KCl, 100 mM LiCl and no added salt, all at 20°C. The second column shows the temperature dependence of the CD spectra in KCl. The third column shows the melting curves in three different ionic conditions.