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. 2019 Aug;25(8):948–962. doi: 10.1261/rna.070417.119

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© 2019 Nasef et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 4. — Topology and length of Cas10-Csm oligoadenylates. (A) The α-³²P-labeled products of each cOA synthesis reaction are incubated either with exonuclease T and PNK enzymes or endonuclease S1. ³²P-labeled degradation products appear at the bottom of the gel in endonuclease reactions migrating in a manner consistent with AMP. Two arrows mark the position of presumed synthesis intermediates which are digested by exonuclease T. (B) MALDI mass spectra of cOA synthesis reaction products are shown along with a diagram depicting product formation.