Table 3.
Mass-spectrometrically identified myelin proteins additionally validated with immuno-based methods
| Protein name | Gene | MS method | Reference | TMD | Cluster | CNS |
|---|---|---|---|---|---|---|
| Annexin A2 | Anxa2 | I,B,S,D,E,wS,wE,wSE | Hayashi et al., 2007 | Descend | N | |
| Band 4.1 protein G | Epb41l2 | B,S,D,E,wS,wE,wSE | Ohno et al., 2006 | P15-UP | N | |
| Carbonic anhydrase 2 | Ca2 | I,D,E | Cammer et al., 1987 | P15-UP | N | |
| Caveolin 1 | Cav1 | S,E,wSE | Mikol et al., 2002 | 1 | Late-UP | N |
| CD151 | Cd151 | S,wS | This article | 4 | Late-UP | N |
| CD81 | Cd81 | D,E,wE,wSE | Ishibashi et al., 2004 | 4 | Late-UP | Y |
| CD9 | Cd9 | S,D,E,wS,wE,wSE | Ishibashi et al., 2004 | 4 | P15-UP | N |
| Cell adhesion molecule 4, NECL4 | Cadm4 | I,S,D,E,wS,wSE | Spiegel et al., 2007 | 1 | P15-UP | Y |
| Cell division control protein 42 | Cdc42 | I,E,wSE | Benninger et al., 2007 | Descend | Y | |
| CKLF-like MARVEL TMD-containing 5 | Cmtm5 | wS,wSE | This article | 4 | P15-UP | N |
| Claudin 19 | Cldn19 | wSE | Miyamoto et al., 2005 | 4 | Late-UP | N |
| CNP | Cnp | I,B,S,D,E,wS,wE,wSE | Matthieu et al., 1980 | P15-UP | Y | |
| Crystallin α2 | Cryab | I,S,D,E,wS,wSE | d'Antonio et al., 2006 | P15-UP | Y | |
| Dihydropyrimidinase related 1 | Crmp1 | E | d'Antonio et al., 2006 | ND | Y | |
| Dystrophin-related protein 2 | Drp2 | E | Sherman et al., 2001 | Late-UP | N | |
| Ezrin | Ezr | I,E | Scherer et al., 2001 | Descend | Y | |
| Fatty acid synthase | Fasn | S,D,E,wS,wE,wSE | Salles et al., 2002 | Late-UP | Y | |
| Gelsolin | Gsn | I,S,D,E,wSE | Gonçalves et al., 2010 | Late-UP | Y | |
| Integrin α6 | Itga6 | I,D,wSE | Nodari et al., 2008 | 1 | P5-UP | N |
| Integrin β1 | Itgb1 | wSE | Feltri et al., 2002 | 1 | Descend | N |
| Moesin | Msn | I,B,S,D,E,wSE | Scherer et al., 2001 | Unchanged | Y | |
| Myelin associated glycoprotein | Mag | S,D,E,wS,wSE | Figlewicz et al., 1981 | 1 | P5-UP | Y |
| Myelin basic protein | Mbp | I,B,S,D,E,wS,wE,wSE | Boggs, 2006 | P15-UP | Y | |
| Myelin protein 2, PMP2 | Pmp2 | I,B,S,D,E,wS,wE,wSE | Trapp et al., 1984 | P15-UP | N | |
| Myelin protein zero, P0 | Mpz | I,B,S,D,E,wS,wE,wSE | Giese et al., 1992 | 1 | P15-UP | Y |
| Myelin proteolipid protein | Plp1 | B,S,D,E,wS,wE,wSE | Garbern et al., 1997 | 4 | P15-UP | Y |
| NDRG1, N-myc downstream regulated | Ndrg1 | I,D,E,wS,wE,wSE | Berger et al., 2004 | Late-UP | Y | |
| Periaxin | Prx | I,B,S,D,E,wS,wE,wSE | Gillespie et al., 1994 | P15-UP | N | |
| Plasmolipin | Pllp | S,E,wS,wSE | Bosse et al., 2003 | 4 | P15-UP | Y |
| Rac1 | Rac1 | I,S,D,E,wSE | Benninger et al., 2007 | Late-UP | Y | |
| Radixin | Rdx | I,E | Scherer et al., 2001 | U-shape | Y | |
| RhoA | Rhoa | I,D,E,wSE | Brancolini et al., 1999 | Unchanged | Y | |
| Sarcoglycan δ | Sgcd | wSE | Cai et al., 2007 | 1 | ND | N |
| Septin 11 | Sept11 | I | Buser et al., 2009 | P5-UP | Y | |
| Septin 2 | Sept2 | I,D,E | Buser et al., 2009 | Descend | Y | |
| Septin 7 | Sept7 | D,E,wSE | Buser et al., 2009 | Descend | Y | |
| Septin 9 | Sept9 | 2D-DIGE | This article | Descend | N | |
| Sirtuin 2, NAD-dependent deacetylase | Sirt2 | I,wSE | Werner et al., 2007 | P15-UP | Y | |
| UDP galactosyltransferase 8 | Ugt8 | wSE | Bosio et al., 1996 | 1 | P15-UP | N |
The proteins identified in peripheral myelin by MS have been compiled according to availability of validation with immuno-based methods. The methods of MS identification are as follows: S, GeLC; wS, wash-GelC; wSE, wash-GeLC-MSE; I, 2D-IEF/SDS-PAGE; B, 2D-16BAC/SDS-PAGE; D, LC-DDA; E, LC-MSE; wE, wash-LC-MSE. One selected reference is given in which presence in peripheral myelin was validated. Additionally given are the number of transmembrane domains (TMDs), the mRNA abundance profile cluster, and whether a protein was also identified by mass spectrometry in CNS myelin according to the compilation by Jahn et al. (2009) (CNS: Y, also identified in CNS myelin; N, not identified in CNS myelin). The complete compendium of MS-identified proteins will be hosted on the author's web page (see Notes). Tetraspan proteins are in bold. ND, mRNA not detected.