Figure 6.

CX3CL1-reduced activation of caspase 3 pathway during pMCAO is A₁R dependent. Brain coronal sections from ischemic rats treated as in Figure 3 were analyzed by Western blot. The same amount of proteins (50 μg) was analyzed for each sample. a, Active form of caspase-3 (17–19 kDa) in the ipsilateral hemisphere of sham-operated (left) or pMCAO rats treated with vehicle or CX3CL1 in the presence of DMSO (control, C; middle) in the presence of DPCPX (right). Results are reported as arbitrary units (a.u.). The inset shows a representative blot: top, active form of caspase-3; bottom: actin, used to normalize signals. b, PARP (116 kDa) in sham-operated (left) or treated rats in the presence or in the absence of DPCPX. Data are expressed as the ratio of PARP in the ipsilateral (I) to the contralateral (C) hemisphere. The inset shows a representative blot. Top, Full-length form of PARP; bottom, AKT, used to normalize signals. Bars indicate the mean ± SE from 6 to 18 rats. Statistical differences are analyzed for each experimental condition for CX3CL1 versus vehicle; *p < 0.05, **p < 0.01 using Student's t test.