Figure 9.
Contralateral axon projection is rescued by electroporation of MKK7 and active-JNK but not by MKK4 or c-Jun. A, Decreased c-Jun phosphorylation. Brain extracts prepared from control and Mkk7flox/flox Nestin-Cre embryos at E18.5 were immunoblotted to detect total c-Jun and phospho-c-Jun. B–E, Reversal of effects of MKK7 disruption. pCAG-NLS-Cre and pCAG-floxed-polyA-mCherry or -GFP were coelectroporated along with MKK7 (0.25 mg/ml) (B), MKK4 (0.25 mg/ml) (C), MKK7-JNK (0.05 mg/ml) (D), or c-Jun (1.0 mg/ml) (E) into the VZ of control and Mkk7flox/flox embryos at E15.5. Brains were fixed at P10 and coronal sections of cortices were immunostained with anti-RFP or GFP antibody. Arrows indicate axons rescued by coelectroporation. Hp, Hippocampus. Scale bar, 1 mm.