Figure 9.

Bmi1 deficiency results in defective DNA damage response. A, WT and Bmi1^−/− MEFs were irradiated at 3 Gy and labeled at different time points (0 min, 30 min, 6 h) after IR with γH2AX antibody. Data are expressed as the percentage of γH2AX-positive cells over total hematoxylin-stained nuclei. Results are mean ± SEM (n = 3, *p < 0.05). B, A working model placing BMI1 as a component of the DNA DSB response machinery. C, BMI1 may be implicated in DNA DSB repair by preferentially interacting with NHEJ proteins in CD133+ GBM cells.