Figure 2.

Norepinephrine and isoproterenol upregulate mFPR2 expression through activation of β₂AR. A, The expression of β adrenergic receptors in N9 cells and mouse primary microglia was examined by RT-PCR. B, C, N9 cells pretreated with 10 μm propranolol (Pro) for 1 h were incubated with or without 1 μm NE for 6 h (B) or 10 μm ISO for 9 h (C), and total RNA was extracted and examined for mFPR2 mRNA level by real-time PCR. Results represent the mean ± SD of three independent experiments. *p < 0.05 versus cells cultured with control medium. ^#p < 0.05, ^##p < 0.01, compared with cells treated with NE or ISO alone.