Figure 3.

Isoproterenol treatment of microglia increases calcium mobilization but not cell migration in response to mFPR2 agonists. A–C, N9 cells were treated with or without 40 μm ISO for 24 h and then loaded with Fura-3-AM and stimulated with different concentrations of W peptide and measured florescence (RFU) (A, B) or examined for mFPR1 and mFPR2 expression by RT-PCR (C). Data shown in A and B are representative of three independent experiments with similar results. Data in C represent the mean ± SD of three independent experiments. **p < 0.01, compared with cells cultured in medium alone. D, E, N9 cells were cultured in the presence or absence of 500 ng/ml LPS or 10 μm ISO at 37°C for 24 h and then examined for migration in response to W peptide (D), and MMK-1 (E). The results are expressed as the mean ± SD of migrated cells in three high-power fields in triplicate samples, **p < 0.01, cell migration in response to W peptide or MMK-1 compared to medium control; ^##p < 0.01, comparison of spontaneous cell migration between N9 cells treated with ISO and LPS. F, Visualization of N9 cell migration in response to W peptide.