Figure 4.

Isoproterenol enhances Aβ₄₂ uptake by microglia. A, E, Primary microglia (A), N9 cells (E) transduced with control lentivirus (Mock), or mFPR2 shRNA-c lentivirus (shRNA-c) were treated with or without 10 μm ISO for 24 h followed by incubation with 10 μm Aβ₄₂ for 30 min and then examined for Aβ uptake with antibody against Aβ (red). Nuclei were stained with Hoechst (blue). Scale bars, 20 μm. Dara are expressed as mean ± SD (n = 2, with duplicate samples in each experiment); **p < 0.01, compared with Aβ uptake by primary microglia or mock transduced N9 cells without ISO treatment; ^##p < 0.01, compared with Aβ uptake by mock transduced N9 cells pretreated with ISO. B–D, mFPR2/293 cells were transduced with control lentiviruses (Mock) or mFPR2 shRNA lentiviruses, mFPR2 mRNA level was examined by RT-PCR (B), and cell migration in response to mFPR2 agonists (C: MMK-1; D: Aβ₄₂) was examined by chemotaxis assay. Data are expressed as mean ± SD (n = 3); *p < 0.05, **p < 0.01, chemotaxis index of mFPR2 shRNA lentivirus transduced cells versus control lentivirus transduced cells (Mock).