Figure 7.

Isoproterenol induces mFPR2 mRNA expression through ERK/p38-NF-κB pathway. A, N9 cells pretreated with or without different concentrations of BAY117082 (BAY) for 1 h were stimulated with 10 μm ISO for 9 h and then examined for mFPR2 expression by RT-PCR. B, C, N9 cells (B) or primary microglia (C) were stimulated with 10 μm ISO for 30 min and then examined for IκBα phosphorylation by Western blot. D, N9 cells pretreated with or without 50 μm PD98059 (PD), 50 μm SB203580 (SB), or 20 μm BAY for 1 h were stimulated with 10 μm ISO for 30 min and then examined for IκBα level by Western blot. All data are mean ± SD (n = 3); *p < 0.05, **p < 0.01, compared with cells cultured in medium alone; ^##p < 0.01, compared with cells treated with ISO alone.