Figure 9.

Taxol prevents ADDL-induced Tau missorting, loss of spines and loss of mitochondria. Primary hippocampal neurons 21DIV were treated with ADDLs (3 h, 5 μm) and 25 nm Taxol. a, Taxol prevents Tau missorting and spine loss, but not 12E8 staining in the dendrites of ADDL-treated neurons. b, Taxol prevents loss of mitochondria (stained with Mitotracker Deep Red). c, Quantification of fraction of missorted cells. Taxol reduces the number of cells displaying Tau missorting. Error bars, SEM from n = 3–4 experiments. **p < 0.01 versus control (one-way ANOVA with post hoc Bonferroni). d, Treatment of neurons with taxol does not affect the number of spines in these conditions. Error bars, SD from n = 10–20 cells per treatment condition. ***p < 0.001 versus control (one-way ANOVA with post hoc Bonferroni). Scale bars, 20 μm.