Figure 7.

Sema1a and PlexA interact genetically with Rho1. A, Overexpression of Sema1a induced the formation of thicker axonal bundles. B, Reducing the dosage of ena by half did not modify the Sema1a-induced hyperfasciculation phenotype. C, Reducing the dosage of Rho1 by half significantly enhanced the Sema1a-overexpression phenotype. D, Reducing the dosage of Rho1 by half in wild-type background did not affect R-cell projection pattern. E, Expressing the dominant-negative form Rho1.N19 in R cells also induced an axonal hyperfasciculation phenotype. F, Knocking down PlexA suppressed the Rho1.N19-induced phenotype. Scale bar, 20 μm.