Figure 2.

Cre-dependent targeting of ADAR2 and GluR2 Q/R site-editing in motor neurons. A, Frequency histogram of editing efficiency at the GluR2 Q/R site in specimens (lysates containing 3 motor neurons) obtained from AR2 mice at 2 months of age (2m; n = 4). Neurons were dissected with a laser microdissector (inset). B, Specimens (n = 116) were collected into four groups depending on the predicted number of ADAR2-deficient neurons in each specimen; the groups of specimens containing 3, 2, 1, and 0 unedited GluR2-expressing neurons were designated as groups 0:3, 1:2, 2:1, and 3:0, respectively. The ADAR2^flox gene and transcripts of the Cre gene and the ADAR2^flox alleles before and after recombination were analyzed for each group by PCR. AHCs expressing unedited GluR2 mRNA (group 0:3) harbored the truncated ADAR2^flox gene and Cre transcripts, whereas AHCs expressing edited GluR2 mRNA (group 3:0) carried the full-length ADAR2^flox gene and did not express Cre. Ctl1, ADAR2^flox/flox mice; Ctl2, VAChT–Cre.Fast mice; AH, anterior horn of the spinal cord.