Figure 3.

Quantitative evaluation of histopathological data. A, Motor neuron numbers in ventrolateral pool of anterior horns, judged by Nissl staining; B, GFAP immunoreactivity, given as pixel values per spinal cord section; C, quantification of CD45 immunoreactivity. In each bar graph, quantification of image data were performed for wr/+ Adam8 ^−/− (black bars), wr/wr Adam8 ^+/− (gray bars), and wr/wr Adam8 ^−/− (white bars). Pixel values from individual images were acquired using NIH ImageJ analysis software. *p < 0.05, ***p < 0.001.