Figure 5.

Perforant path preferentially recruits FS interneurons at physiological temperature and in cell-attached mode. A, Five hundred millisecond clips of simultaneously recorded current-clamp responses of a GC (left) and a FS interneuron (right) to a 5 Hz stimulus train delivered to the outer molecular layer at 35°. B, Same as A, but for 10 Hz. Significantly more spikes were recruited in FS interneurons compared with GCs (p ≤ 0.021, n = 4, paired t test). Asterisks mark recruited doublets. C, Simultaneously recorded cell-attached responses of FS (top) and GC (bottom) in control conditions (left) and 10 μm bicuculline (right) to 10 Hz stimulus train delivered to the outer molecular layer. Significantly more spikes were recruited in FS INs compared with GCs (p ≤ 0.008, paired t test, n = 6 pairs). Note that FS INs fire bursts for perforant path input in both whole-cell and cell-attached recording modes.