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. 2019 Jul 10;146(13):dev166504. doi: 10.1242/dev.166504

Fig. 3.

Fig. 3.

Expression of mon1 in the OPNs is sufficient to rescue lethality and ovarian defects in mon1Δ181. (A,B) Pan-neuronal expression of UAS-mon1:HA using C155-GAL4 in mon1Δ181 (B) rescues the ‘small ovary’ phenotype (A). (C,D) Expression of UAS-mon1:HA in a small subset of ∼137 neurons, using tdc2-GAL4 (D, tdc2-GAL4, mon1Δ181/mon1Δ181; UAS-mon1:HA) also rescues the ovary phenotype in mon1Δ181(C). (E) The short lifespan of mon1Δ181 adults is rescued by expression of the gene in the tdc2 domain (tdc2-GAL4, mon1Δ181>; UAS-mon1:HA). The animals live for more than 30 days compared with the mutants that fail to survive beyond 10 days. (F) Expression of mon1:HA in OPNs also rescues the decrease in ovariole number observed in mon1Δ181 animals (35±0.433 in wild type versus 21.3±1.12 in mon1Δ181 versus 34.6±0.4 in tdc2-GAL4, mon1Δ181>UAS-mon1:HA). (G) The ‘stalling’ defect in mon1Δ181 ovarioles is rescued by expression of mon1:HA in the OPNs. More than a 4-fold increase is seen in the percentage of stage 9-10 egg chambers with respect to the ‘GAL4’ control (2.91±1.23% in mutant ‘GAL4’ control versus 13.9±1.24% in ‘rescue’) and a 2-fold increase with respect to the ‘UAS’ control (6.8±0.9% in ‘UAS’ mutant control versus 13.9±1.24% in tdc2-GAL4, mon1Δ181>UAS-mon1:HA animals). A 10- and 5-fold increase in the number of eggs is observed with respect to the GAL4 and UAS controls, respectively (∼0% in ‘GAL4’ control versus 2.38±0.6% in ‘UAS’ control versus 10.05±0.5% in tdc2-GAL4, mon1Δ181>UAS-mon1:HA animals). (H) A comparison of the number of eggs laid per day by w1118, C155-GAL4, mon1Δ181>UAS-mon1:HA and tdc2-GAL4, mon1Δ181>UAS-mon1:HA animals over 5 days. On average, wild-type and tdc2-GAL4, mon1Δ181>UAS-mon1:HA animals are seen to lay comparable numbers of eggs, with the average number of eggs being 85-95 by the 4th day. In comparison, the number of eggs produced by C155-GAL4, mon1Δ181>UAS-mon1:HA animals remains constant in the range of 65-70. N=number of animals, n=number of ovarioles; ***P<0.001, **P<0.01; *P<0.05; two-way ANOVA.