Figure 2.

Acute expression of the PKA inhibitor, PKI, in the mushroom bodies at old age suppresses AMI. A, Flies were fed RU486 from 1 to 18 d of age (1–18) or fed without RU486 (−). Memory was tested at 1 or 18 d of age. Continuous RU486 feeding suppresses AMI in PKI;;MBS flies. N = 6–12. B, Flies were fed RU486 from 18 to 20 d of age (18–20) or fed without RU486 and memory was tested at 1, 18, or 20 d of age. Acute feeding of RU486 at old age suppresses AMI in PKI;;MBS flies. N = 8–12. C, Flies were fed RU486 from 1 to 18 or 1 to 20 d of age or fed without RU486 and memory was tested at 1, 18, or 20 d of age. AMI is restored in 20-d-old PKI;;MBS flies when RU486 feeding is stopped at 18 d of age. N = 7–12. All data are expressed as means ± SEM. Data were analyzed as described in the legend to Figure 1.