Table 1.
Firing pattern | Cluster firing | Fast firing |
Burst firing | Slow firing | |
---|---|---|---|---|---|
Large sag | No sag | ||||
N (% of total number) | 13 (19.7%) | 12 (18.2%) | 19 (28.8%) | 11 (16.7%) | 11 (16.7%) |
Vrest (mV) | −53.0 ± 1.6 | −50.3 ± 1.0a | −51.5 ± 1.3 | −52.0 ± 0.9a | −49.5 ± 1.0 |
Proportion of spontaneous firing | 46.2% | 50.0% | 58.8% | 54.5% | 18.2% |
Mean frequency (Hz) | 2.8 ± 0.7 | 3.7 ± 1.3 | 4.6 ± 1.2 | 2.5 ± 0.6 | 3.5 ± 0.5 |
Range (Hz) | 1–6 | 1–9 | 1–10 | 1–5 | 3–4 |
Mean firing rate (Hz) | 8.8 ± 1.2b,c | 15.8 ± 1.6d,e,f | 13.6 ± 1.8d,e,f | 9.1 ± 0.9b,c | 5.3 ± 0.3b,c |
FINITIAL (Hz) | 12.7 ± 2.2 | 18.3 ± 2.3f | 15.8 ± 1.9f | 12.3 ± 1.6 | 5.9 ± 0.6b,c |
FFINAL (Hz) | 6.2 ± 1.3b,c | 15.4 ± 2.1d,e,f | 13.4 ± 1.7d,e,f | 7.7 ± 1.0b,c | 4.1 ± 0.6b,c |
Spike frequency accomodation (%) | 51.8 ± 10.4b,c | 15.4 ± 6.2d | 13.4 ± 3.8d | 37.3 ± 9.1 | 27.3 ± 12.4 |
Spike threshold (mV) | −41.2 ± 0.9 | −43.1 ± 1.0 | −44.1 ± 1.2f | −43.9 ± 1.3f | −38.9 ± 1.1c,e |
Spike amplitude (mV) | 78.1 ± 2.9 | 80.7 ± 2.0 | 79.8 ± 2.2 | 81.9 ± 3.4 | 76.6 ± 2.2 |
Spike half-width (ms) | 1.3 ± 0.1 | 1.3 ± 0.1 | 1.3 ± 0.1 | 1.2 ± 0.1f | 1.7 ± 0.1e |
AHP amplitude (mV) | 7.5 ± 1.5 | 5.9 ± 0.9 | 8.1 ± 0.6 | 4.9 ± 0.6 | 7.4 ± 0.9 |
AHP duration (ms) | 253.2 ± 39.9 | 262.8 ± 31.4 | 284.5 ± 34.1 | 292.6 ± 20.8a | 318.9 ± 31.1 |
Sag amplitude (mV) | 6.4 ± 1.3b | 16.1 ± 1.4c,d,e,f | 4.6 ± 0.7b | 3.2 ± 1.1a,b | 4.9 ± 1.4b |
Rebound spike delay (ms) | 124.5 ± 42.6 | 221.7 ± 87.1 | 94.2 ± 18.4 | 74.7 ± 7.9 | 74.3 ± 6.1a |
Sag activation time (ms) | — | 131.1 ± 21.3 | — | — | — |
Sag decay constant (ms) | — | 176.9 ± 19.5 | — | — | — |
Subthreshold oscillation frequency (Hz) | 17.8 ± 2.4 | — | — | — | — |
Intracluster/burst frequency (Hz) | 15.8 ± 2.2 | — | — | 58.6 ± 11.4 | — |
No. of spikes per cluster/burst | 4.9 ± 0.6 | — | — | 2.3 ± 0.3 | — |
LTS amplitude (mV) | — | — | — | 15.3 ± 1.2a | — |
LTS duration (ms) | — | — | — | 162.7 ± 39.5 | — |
Values are given as mean ± SE. Statistically significant differences (p < 0.05) are indicated as follows: aversus GAD65-eGFP-(+) cells with a similar firing pattern bversus fast-firing cells with large sag; cversus fast-firing cells with no sag; dversus cluster-firing cells; eversus burst-firing cells; fversus slow-firing cells.