Figure 5.

Ddc- and serotonin-labeled cells in larval and adult brains do not overlap with the DILP2 neurons. A–L, Projections of confocal Z-stacks of a wild-type Drosophila adult brain expressing mCD8GFP with Dilp2GAL4 and immunostained with anti-serotonin antibody (A, E, I), anti-Ddc antibody (B, F, J) and anti-GFP antibody (C, G, K). D, H, and L are a merge of the corresponding images with anti-serotonin labeling in blue, anti-Ddc labeling in red, and anti-GFP labeling in green. Successive rows are higher-magnification images. Top and bottom blue arrows in E indicate serotonin-positive varicosities near the DILP2 neurons and in the subesophageal ganglia, respectively. Blue arrowheads in I and red arrowheads in F and J mark cells that are Ddc and serotonin positive and send out processes centrally toward the subesophageal ganglia (red arrows in F, J) that seem to intermingle with the processes sent out by the DILP2 neurons (green arrows in C, G, K). Green arrowhead in C and white arrowheads in D, H mark the DILP2 neurons. Scale bars: A–H, 50 μm; I–L, 20 μm.