Figure 5.

Aβ expression increases the size and heterogeneity of synaptic vesicles and causes accumulation of presynaptic vacuoles in an age-dependent manner. Presynaptic sections from control (A), Aβ_42×2 (C), and Aβ_arc (B, D) flies at the age of 21 d. The black arrowhead indicates the large synaptic vesicles, and * indicates the vacuole (diameter of >80 nm). E, Quantification and accumulative probability of synaptic vesicle diameters at the age of 3, 9, and 21 d. n = 600–800. F, The histogram of log value of the area of vesicles and vacuoles in square nanomolar. Note the second peak (indicated by arrow) in Aβ_arc flies at the age of 21d. Scale bars, 200 nm.