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. 2010 Nov 10;30(45):15196–15209. doi: 10.1523/JNEUROSCI.0934-10.2010

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Copyright © 2010 the authors 0270-6474/10/3015196-14$15.00/0

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Figure 3. — Multiple CF innervation by ascending CF branches after induction of GluRδ2 ablation. Each panel shows parasagittal cerebellar sections subjected to dextran Alexa-594 tracer labeling for CFs (DA-594; red) and immunofluorescence for calbindin (calb; blue) and VGluT2 (green) in GluRδ2^flox/flox at 2 weeks after RU-486 administration (A) and GluRδ2^flox/CrePR mice at 2 weeks (B), 8 weeks (C), and 24 weeks (D) after RU-486 administration. Immunoreaction for calbindin is pseudocolored in blue (A₁–D₁) or brown (A₂–D₂, A₃–D₃). CF-a indicates the CF mainly innervating a PC dendrite of interest (PCD-a). In GluRδ2^flox/CrePR mice at 8 and 24 weeks, PCD-a is multiply innervated by both main CF-a and additional CF-b (arrows). Scale bars, 10 μm.