Table 1.
Normal GC proliferation, differentiation, cell death, and blood vessel density in VEGF∂/∂ and VEGF188/188 mice
| WT | VEGF∂/∂ | VEGF188/188 | p value | |
|---|---|---|---|---|
| P27+ GCs—lower EGL (P10) (p27+ GCs/100 μm EGL) | 38.5 ± 3.0 | 55.3 ± 5.6 | p < 0.05 | |
| Pax6 density (P10) (Pax6+ GCs/103 μm2 EGL) | 26.1 ± 4.1 | 27.6 ± 3.4 | p = NS | |
| GCP proliferation—upper EGL (P10) (Ki67+ GCs/100 μm EGL) | 33.6 ± 3.0 | 37.8 ± 1.5 | p = NS | |
| GCP proliferation (P10) (BrdU+ GCs/100 μm EGL) | 26.5 ± 1.5 | 23.3 ± 1.3 | p = NS | |
| GCP proliferation (P10) (PH3+ GCs/mm EGL length) | 13.2 ± 1.4 | 14.5 ± 2.2 | p = NS | |
| GC apoptosis (P10) (act-casp-3+ GCs/105 μm2 EGL) | 10.9 ± 0.6 | 10.9 ± 0.9 | p = NS | |
| Blood vessel density (P12) (CD31+ area, as % of total cerebellum area) | 5.7 ± 0.8 | 4.8 ± 0.8 | p = NS | |
| P27+ GCs—lower EGL (P12) (p27+ GCs/100 μm EGL) | 26.2 ± 1.6 | 17.6 ± 2.0 | p < 0.05 | |
| Pax6 density (P12) (Pax6+ GCs/103 μm2 EGL) | 29.7 ± 0.7 | 27.1 ± 1.3 | p = NS | |
| GCP proliferation—upper EGL (P12) (Ki67+ GCs/100 μm EGL) | 12.0 ± 1.4 | 12.0 ± 1.0 | p = NS | |
| GCP proliferation (P10) (BrdU+ GCs/100 μm EGL area) | 19.4 ± 0.5 | 19.3 ± 1.7 | p = NS | |
| GC proliferation (P10) (PH3+ GCs/mm EGL length) | 17.8 ± 2.5 | 18.8 ± 1.4 | p = NS | |
| GC apoptosis (P10) (act-casp-3+ GCs/105 μm2 EGL) | 10.6 ± 0.8 | 10.6 ± 0.7 | p = NS | |
| Blood vessel density (P12) (CD31+ area, as % of total area) | 4.2 ± 0.6 | 4.1 ± 0.6 | p = NS |