Table 2.

Ectopic expression of VEGF or inhibition of Flk1 in cerebellar slices inhibits GC migration

Area	Ectopic VEGF overexpression	αFlk1	PTK
1	+14.0%**	+16.2%**	+30.0%**
2	−4.0%	+2.7%	−6.0%
3	−18.0%**	−19.31%**	−48.0%**
4	−22.0%**	−33.78%**	−66.0%**
5	−26.0%**	ND	ND

Area 1–5 represent equally divided areas of the cerebellar cortex, from the EGL/ML border to the IGL (Fig. 4F), and the number of GFP⁺ GCs that migrated into each of these areas was counted in control cerebellar slices, in slices coelectroporated with the VEGF expression vector and a GFP expression vector, or in slices electroporated with a GFP expression vector and treated with αFlk1 antibody or the VEGF receptor inhibitor PTK. The observed number of GCs in each area in the treated slices was then statistically compared, using binomial nonparametric statistics, with the expected number of GCs, as detected in control slices. The difference between the observed versus expected number of cells in each area was expressed as a percentage of the expected number of cells. Data of PTK and αFlk1 treatment in area 5 could not be determined (ND) because of the low amount of cells present in this area.

**p ≤ 0.001 versus corresponding controls.