Table 3.
Intra-/inter-day precision and accuracy for the determination of the components in rat plasma.
| Analytes | Spiked Concentration (ng/mL) |
Precision (%) | Accuracy (%, Mean ± SD) | ||
|---|---|---|---|---|---|
| Intra-day | Inter-day | Intra-day | Inter-day | ||
| Baicalin | 2.1 | 6.54 | 2.96 | 85.12 ± 5.82 | 90.10 ± 9.84 |
| 25.0 | 5.82 | 10.87 | 90.73 ± 7.80 | 87.45 ± 4.95 | |
| 2625 | 10.76 | 11.71 | 91.19 ± 8.37 | 94.14 ± 7.30 | |
| Baicalein | 2.0 | 6.60 | 5.41 | 95.19 ± 8.95 | 92.61 ± 7.28 |
| 25.0 | 11.72 | 7.98 | 96.46 ± 8.17 | 98.19 ± 6.89 | |
| 575 | 6.86 | 4.24 | 100.15 ± 9.36 | 105.85 ± 7.94 | |
| Wogonoside | 2.0 | 3.45 | 14.31 | 87.12 ± 5.84 | 102.19 ± 9.78 |
| 25.0 | 7.85 | 7.54 | 90.73 ± 7.91 | 85.96 ± 5.85 | |
| 1875 | 7.19 | 0.77 | 101.14 ± 5.38 | 98.89 ± 7.23 | |
| Wogonin | 2.0 | 2.91 | 5.97 | 96.45 ± 5.89 | 88.77 ± 4.93 |
| 25.7 | 4.53 | 14.11 | 92.76 ± 6.89 | 94.75 ± 5.73 | |
| 136.7 | 2.06 | 9.92 | 95.71 ± 8.78 | 90.50 ± 3.35 | |
| Berberine | 0.25 | 3.92 | 13.28 | 88.19 ± 6.59 | 88.14 ± 5.05 |
| 2.50 | 9.48 | 2.99 | 99.45 ± 8.84 | 85.79 ± 8.78 | |
| 25.0 | 1.29 | 12.25 | 100.89 ± 8.73 | 91.16 ± 4.38 | |
| Palmatine | 0.25 | 3.75 | 10.54 | 85.12 ± 5.81 | 95.19 ± 8.95 |
| 2.50 | 7.36 | 9.66 | 90.89 ± 7.93 | 106.49 ± 8.19 | |
| 25.0 | 6.28 | 0.78 | 108.78 ± 4.73 | 110.14 ± 9.24 | |
| Jatrorrhizine | 0.25 | 3.55 | 13.06 | 104.12 ± 7.46 | 87.02 ± 6.24 |
| 2.50 | 7.18 | 6.57 | 92.57 ± 6.65 | 90.64 ± 5.93 | |
| 25.0 | 4.38 | 0.86 | 98.10 ± 4.35 | 96.18 ± 4.97 | |