Table 5.
Stability of the components in rat plasma under a variety of storage and process conditions.
| Analyte | Spiked Concentration (ng/mL) |
RSD% | |||
|---|---|---|---|---|---|
| Freeze-thaw cycles | Short-term stability | Long-term stability | Auto-sampler stability | ||
| (three freeze-thaw cycles) | (room temperature, 4 h) | (-20°C, 30 d) | (4°C, 12 h) | ||
| Baicalin | 2.1 | 13.86 | 10.53 | 5.63 | 6.93 |
| 25.0 | 5.34 | 4.56 | 5.91 | 8.72 | |
| 2625 | 10.64 | 8.45 | 1.25 | 2.16 | |
| Baicalein | 2.0 | 8.17 | 6.48 | 3.99 | 12.79 |
| 25.0 | 7.89 | 5.78 | 6.23 | 7.89 | |
| 575 | 3.73 | 6.78 | 5.69 | 11.36 | |
| Wogonoside | 2.0 | 5.54 | 3.91 | 2.46 | 7.88 |
| 25.0 | 10.35 | 4.99 | 14.16 | 2.52 | |
| 1875 | 11.95 | 11.56 | 11.99 | 8.38 | |
| Wogonin | 2.0 | 4.25 | 8.72 | 1.96 | 4.53 |
| 25.7 | 7.11 | 12.12 | 3.79 | 3.73 | |
| 136.7 | 13.62 | 10.56 | 2.88 | 4.79 | |
| Berberine | 0.25 | 12.30 | 7.63 | 3.73 | 7.82 |
| 2.50 | 11.01 | 8.43 | 12.19 | 10.13 | |
| 25.0 | 14.52 | 4.96 | 13.13 | 7.40 | |
| Palmatine | 0.25 | 15.75 | 8.45 | 13.83 | 9.93 |
| 2.50 | 11.82 | 8.31 | 11.98 | 6.67 | |
| 25.0 | 10.07 | 4.97 | 2.54 | 7.99 | |
| Jatrorrhizine | 0.25 | 11.20 | 7.54 | 2.86 | 7.87 |
| 2.50 | 11.16 | 6.08 | 2.11 | 11.69 | |
| 25.0 | 14.82 | 9.05 | 1.31 | 4.09 | |