Table 1.
Summary of the results of the initial mutation test.
| Initial Mutation Test (Plate Incorporation Test) | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Concentrations (μg/plate) | Salmonella typhimurium tester strains | Escherichia coli | ||||||||||||||||||
| TA 98 | TA 100 | TA 1535 | TA 1537 | WP2uvrA | ||||||||||||||||
| -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | |||||||||||
| Mean values of revertants per plate Mutation rate (MR) | Mean | MR | Mean | MR | Mean | MR | Mean | MR | Mean | MR | Mean | MR | Mean | MR | Mean | MR | Mean | MR | Mean | MR |
| Untreated Control | 15.7 | 1.09 | 15.3 | 0.82 | 62.3 | 0.88 | 77.7 | 0.77 | 8.3 | 0.89 | 9.7 | 1.21 | 7.7 | 0.92 | 6.0 | 1.06 | 34.3 | 0.75 | 48.3 | 1.06 |
| DMSO Control | 13.7 | 1.00 | 16.0 | 1.00 | – | – | 92.0 | 1.00 | – | – | 9.7 | 1.00 | 6.0 | 1.00 | 7.0 | 1.00 | – | – | 44.0 | 1.00 |
| Ultrapure Water Control | 14.3 | 1.00 | 18.7 | 1.00 | 70.7 | 1.00 | 100.7 | 1.00 | 9.3 | 1.00 | 8.0 | 1.00 | 8.3 | 1.00 | 5.7 | 1.00 | 46.0 | 1.00 | 45.7 | 1.00 |
|
| ||||||||||||||||||||
| TEST ITEM | ||||||||||||||||||||
|
| ||||||||||||||||||||
| 5000 | 16.3 | 1.14 | 24.3 | 1.30 | 73.7 | 1.04 | 89.7 | 0.89 | 9.3 | 1.00 | 8.0 | 1.00 | 9.0 | 1.08 | 7.3 | 1.29 | 37.7 | 0.82 | 44.3 | 0.97 |
| 1600 | 14.0 | 0.98 | 19.0 | 1.02 | 68.3 | 0.97 | 96.7 | 0.96 | 10.0 | 1.07 | 9.0 | 1.13 | 7.0 | 0.84 | 4.0 | 0.71 | 42.0 | 0.91 | 54.3 | 1.19 |
| 500 | 13.7 | 0.95 | 23.3 | 1.25 | 85.3 | 1.21 | 108.0 | 1.07 | 10.3 | 1.11 | 10.7 | 1.33 | 9.0 | 1.08 | 4.7 | 0.82 | 48.7 | 1.06 | 47.3 | 1.04 |
| 160 | 16.7 | 1.16 | 18.7 | 1.00 | 79.3 | 1.12 | 113.7 | 1.13 | 11.0 | 1.18 | 11.0 | 1.38 | 5.7 | 0.68 | 5.0 | 0.88 | 44.7 | 0.97 | 51.0 | 1.12 |
| 50 | 11.3 | 0.79 | 17.0 | 0.91 | 68.7 | 0.97 | 101.3 | 1.01 | 10.3 | 1.11 | 11.7 | 1.46 | 7.3 | 0.88 | 11.3 | 2.00 | 40.0 | 0.87 | 44.3 | 0.97 |
| 16 | 19.7 | 1.37 | 25.0 | 1.34 | 72.7 | 1.03 | 101.7 | 1.01 | 10.7 | 1.14 | 11.0 | 1.38 | 7.0 | 0.84 | 6.7 | 1.18 | 35.7 | 0.78 | 55.0 | 1.20 |
|
| ||||||||||||||||||||
| POSITIVE CONTROLS | ||||||||||||||||||||
|
| ||||||||||||||||||||
| NPD (4 μg/plate) | 387.3 | 28.34 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SAZ (2 μg/plate) | – | – | – | – | 848.0 | 12.00 | – | – | 853.3 | 91.43 | – | – | – | – | – | – | – | – | – | – |
| 9AA (50 μg/plate) | – | – | – | – | – | – | – | – | – | – | – | – | 245.3 | 40.89 | – | – | – | – | – | – |
| MMS (2 μL/plate) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 1053.3 | 22.90 | – | – |
| 2AA (2 μg/plate) | – | – | 1757.3 | 109.83 | – | – | 2077.3 | 22.58 | – | – | 171.3 | 17.72 | – | – | 130.7 | 18.67 | – | – | – | – |
| 2AA (50 μg/plate) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 241.0 | 5.48 |
MR, mutation rate; DMSO, dimethyl sulfoxide; NPD, 4-Nitro-1,2-phenylenediamine; SAZ, sodium azide; 9AA, 9-aminoacridine; MMS, methyl methanesulfonate; 2AA, 2-aminoanthracene.
Remarks. Ultrapure water was applied as vehicle of the test item and the positive control substances: SAZ and MMS; and the DMSO was applied as vehicle for positive control substances: NPD, 9AA, and 2AA. The mutation rate of the test item, SAZ, MMS, and untreated control refers to the ultrapure water; the mutation rate of NPD, 9AA, and 2AA refers to DMSO.