Figure 6. Combination therapy reduced Treg cells and activated CD8⁺ T cells and in splenocytes.

(A, B) Representative cytometric dot plots and quantification of CD4⁺CD25⁺FoxP3⁺ Treg cells. (C, D) Representative cytometric dot plots and quantification of CD8⁺CD69⁺ T cells. (E, F) Representative cytometric dot plots and quantification of CD8⁺IFNγ⁺ T cells. (G) Percentage of CD4⁺CD69⁺ cells. (H) Percentage of CD4⁺IFNγ⁺ T cells. (I) Quantification of IFNγ in the supernatant of cultured splenocytes after stimulation by mesothelin peptides by ELISA (n = 5).