Figure 2.
Selectivity of HyCL-3 ad HyCL-4-AM. Response of (A) 20 μM HyCL-3 in the (blue trace) absence and (red trace) presence of 200 μg mL−1 rat liver microsomes (RLM) and 50 μM NADPH and (B) 20 μM HyCL-4-AM in the (blue trace) absence and (red trace) presence of 200 μg mL−1 rat liver microsomes (RLM) and 50 μM NADPH. Response of (C) 20 μM HyCL-3 and (D) 20 μM HyCL-4-AM to the indicated analytes from 0 to (red bars) 50 at 10 min increments. All experiments were performed in 10 mM PBS (pH 7.4) containing ≤1% DMSO. Error bars are ± SD.