Figure 3.

Distribution patterns of GFP signals in tangential sections of the barrel cortex. GFP was expressed in layer 2/3 neurons using in utero electroporation, and 50 μm cortical tangential sections were prepared at P15. The sections corresponding to layer 4 were used for further analyses. A, A low-magnification image of the entire tangential section. Note that whisker-related patterns are visible in the barrel cortex, whereas surrounding areas do not show apparent patterns. Arrowhead, Injection site of plasmid; asterisk, olfactory bulb. Scale bar, 2 mm. B, C, Higher-magnification images of the barrel cortex. Scale bars, 200 μm. D–F, Tangential sections were immunostained with anti-VGLUT2 antibody. GFP-positive axons (D), VGLUT2-positive TCAs (E), and a merged image (F) are shown. Note that GFP signals are predominantly located in the septal regions (arrows). Scale bars, 200 μm.