Figure 8.

The effect of follicle cauterization on whisker-related patterns of barrel nets during development. A, The experimental procedure for examining the effect of follicle cauterization. In utero electroporation was performed at E15.5, and follicles of row C whiskers were cauterized at one of the indicated time points. Tangential sections of the cerebral cortex were prepared at P15 and stained with anti-VGLUT2 antibody. B, Distribution patterns of VGLUT2 immunoreactivity and GFP signals in tangential sections. Follicle cauterization at P1 or P3 resulted in reorganization of the barrels corresponding to row C (arrowheads), and whisker-related patterns of barrel nets were altered accordingly (arrows). Whisker-related patterns of barrels and barrel nets were not altered by follicle cauterization at P6. Scale bar, 200 μm.