Figure 6.

Effect of quinpirole on H-FABP-overexpressing SHSY-5Y human neuroblastoma cells. A, Schematic diagrams of H-FABP sense (top) and antisense (bottom) orientation plasmids in pcDNA3. H-FABP cDNA is the open reading frame of Mus musculus fatty acid binding protein 3 (NM_010174). B, Representative immunoblots of cell extract proteins from treated (Quin.) and control (D.W.) cells probed with anti-pERK, anti-D2LR, H-FABP and anti-ERK (conventional) antibodies. C, Quantitative analysis of ERK activity was performed by measuring phosphorylated ERK immunoreactivity. After treatment of cells with 10 μm quinpirole or distilled water (D.W.) for 10 min in standard medium, cell extracts (20 μg) were prepared and subjected to immunoblot analysis. Each bar represents the mean ± SEM. **p < 0.01 versus distilled water-treated group; ^## p < 0.01 versus antisense with quinpirole-treated group.