Figure 5.

The depressant effect of kainate on GPSCs involves a G-protein-mediated mechanism. A, Summary plot showing the effects of UBP 302 on the peak amplitude of GPSCs in controls (open symbols) and after overnight treatment with PTx (closed symbols). The insets above the graph represent average traces obtained in the presence of GYKI 52466 and GYKI 52466 plus UBP 302. B, As in A, but in the presence of the glutamate scavenger showing that also the effect of the scavenger is dependent on G-protein. C, Application of baclofen (20 μm; bar) induced an outward currents associated with a reduction of spontaneous synaptic events (top trace). Application of baclofen to a cell recorded with a patch pipette containing GDPβS failed to produce an outward current but was still able to depress the ongoing synaptic activity (bottom trace). D, E, GDPβS into the intrapipette solution did not affect UBP 302-induced GPSCs facilitation. D, Representative traces. E, Summary plot from five cells. Error bars indicate SEM.