Figure 6.

Blocking PLC with U73122 reveals the ionotropic action of GluK1 on MF-GPSCs. A, Averaged traces of MF-GPSCs evoked in a CA3 principal cell in the presence of U73122 (10 μm; Control) and U73122 plus UBP 302 (10 μm). B, Summary plot showing the mean amplitude of GPSCs obtained in six cells in the presence of U73122 before and during application of UBP 302 (bar). The amplitude of synaptic responses is normalized to those obtained before UBP 302 (dashed line). In the presence of UBP 302, the amplitude of the synaptic responses was significantly different from controls (p = 0.01). C–F, As in A and B, but in the presence of ATPA (1 μm) (C, D) and the glutamate scavenger (GPT plus pyruvate) (E, F). Note that both the ATPA-induced potentiation and the scavenger-induced depression of GPSCs amplitude were significantly different from controls (p = 0.01 and p < 0.001, respectively). Error bars indicate SEM.