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. 2010 Feb 3;30(5):1766–1776. doi: 10.1523/JNEUROSCI.4962-08.2010

Figure 6.

Figure 6.

png-1 can act from epithelial cells or neurons to regulate VC axon branching. A, Cell-specific promoters were used to drive png-1 expression in epithelial cells or neurons. Constructs were injected along with odr-1::RFP injection marker into png-1(cy9); cyIs4[cat-1p::GFP] animals to generate multiple independent extrachromosomal transgenic lines. Lines expressing png-1 from pan-epithelial (col-10, ajm-1) or pan-neuronal (rgef-1) promoters rescue VC4-5 branching defects. png-1 expression in vulval cells (sqv1, sqv-3) or VC4-5 (cat-1) is sufficient for rescue. B, C, DVB axon overextension (B) and branching (C) defects are rescued with both pan-epithelial (ajm-1) and pan-neuronal (rgef-1) expressed png-1. Controls are empty vectors. Error bars denote SE of proportion; n = 62–200 animals. *p < 0.05, **p < 0.01, ***p < 0.001, t test.