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. 2010 Feb 10;30(6):2017–2024. doi: 10.1523/JNEUROSCI.5693-09.2010

Figure 2.

Figure 2.

Generation and validation of DAGLβ−/− mice. a, DAGLβ knock-out mice were generated from a Lexicon OmniBank ES cell clone OST195261, which contains a gene trap cassette insertion in the first exon of the mouse DAGLβ gene. LTR, Long terminal repeat; NEO, neomycin gene; pA, polyadenylation sequence; SV40tpA, SV40 triple polyadenylation sequence; PGK, phosphoglycerate kinase-1 promoter; BTK, Bruton tyrosine kinase. b, TaqMan analysis for DAGLβ mRNA levels in the cortex (CTX), cerebellum (Cb), spinal cord (SC), and liver (LI) from wt (+/+), DAGLβ+/− (+/−), and DAGLβ−/− (−/−) mice (n = 4/genotype). DAGLβ expression is normalized as the percentage of GAPDH levels. Error bars indicate SEM. c, Western blot analysis of cerebellum tissues from wt (WT), DAGLα−/− (α−/−), and DAGLβ−/− (β−/−) mice demonstrates the absence of DAGLβ protein in DAGLβ−/− mice.