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Expression of 5-HT1AR, 5-HT2AR, and 5-HT2CR mRNAs in PV-positive neurons. A–C, Colocalization of the FSi marker parvalbumin (PV) with 5-HT1AR (A), 5-HT2AR (B), and 5-HT2CR (C) mRNAs by double ISH (MOs, n = 3 rats each). Note a higher background on the latter ISH, consequence of a weak expression of the signal. D, Colocalization of 5-HT2CR mRNAs with the pyramidal neuron marker VGluT1. Examples of double-labeled neurons are marked with white arrows. Scale bars are 20 μm.
