Figure 6.
Signaling cascades involved in the stimulation of glucose utilization by Aβ. Astrocytes were stimulated with 25 μm Aβ25-35 for 24 h and glucose utilization was determined using the [3H]-2-DG technique. When indicated, U0126 (20 μm), SB202190 (20 μm), and LY294002 (10 μm), inhibitors of the p42/44 MAP kinase, p38 MAP kinase and the PI3-kinase cascades, respectively, were added 30 min before Aβ25-35 and maintained throughout the whole incubation. Results are expressed as percentage of control (Ctrl) values (60.9 ± 5.7 fmol/dish) and are means ± SEM of at least eight determinations from three independent experiments. Data were statistically analyzed with ANOVA followed by Bonferroni's test (***p < 0.001 vs respective basal conditions, Ctrl, U0126, and SB202190; ns, not significantly different from LY294002; U0126, SB202190, and LY294002, not significantly different from Ctrl).