Figure 3.

Rbpj is required for maintenance of neural stem/progenitor cells in the late embryonic telencephalon. A–N, Histological analysis was done with control (A–G) and Rbpj-conditional knock-out (H–N) mice at E18.5. Tamoxifen was administered to pregnant mothers at E14.5. In Rbpj-mutant embryos, the number of BrdU-incorporated dividing progenitors was significantly reduced in the dorsal and lateral regions of the SVZ of the lateral ventricle (H–J) compared with controls (A–C). However, in the ventral region, the number of BrdU-incorporated cells increased in Rbpj-mutant embryos (H, K), compared with controls (A, D). Neurons were located outside of the ventricular zone in control mice (E–G), whereas numerous DCX-positive neurons were observed in the ventricular zone of Rbpj-mutant mice (L–N), indicating premature neurogenesis.