Figure 4.

Increase in the number of transit-amplifying cells and neuroblasts in the Rbpj-mutant adult brain. A–N, Coronal sections of the SVZ of the lateral ventricles of control (A–G) and Rbpj-conditional knock-out (H–N) mice that were treated with tamoxifen 3 weeks before. BrdU was administered for 2 h before mice were killed. The number of BrdU-incorporated cells in the SVZ significantly increased in Rbpj-mutant mice (H–K) compared with controls (A–D). The number of Mash1⁺ transit-amplifying cells also increased in Rbpj-mutant mice (L, M) compared with controls (E, F). Boxed regions in panels E and L are enlarged in F and M, respectively. Neurogenesis (DCX+) was enhanced in Rbpj-mutants (N) compared with controls (G).