Figure 2.

Increased hippocampal neurogenesis in Gsn^−/− mice. A, B, Hippocampal neurogenesis was assessed in 4- to 5-month-old mice after a 4 week delay between BrdU injections and killing. Whereas the number of BrdU⁺ cells was strongly increased in Gsn^−/− animals (more than threefold), neuronal versus glial fate commitment did not differ significantly between genotypes. B, Confocal image illustrating newly generated neuron in the dentate gyrus of Gsn^−/− mouse. Green, Neuronal marker NeuN. Red, BrdU. Blue, Astrocytic marker S100β. C, D, The number of DCX- and CR-expressing cells was used as a surrogate marker to assess hippocampal neurogenesis in 14- to 16-month-old mice. Representative images of DCX immunoreactivity (C) and CR immunoreactivity (D) in the hippocampal dentate gyrus of Gsn^−/− mice and wild-type controls. Scale bar: (in D) B, 23 μm; C, D, 50 μm.