Table 1.
Gene | Sense | Antisense |
---|---|---|
SRF | GCTACACGACCTTCAGCAAGAG | CAGGTAGTTGGTGATGGGGAAG |
rodAct (β-actin) | ACCCACACTGTGCCCATCTA | GCCACAGGATTCCATACCCA |
GAPDH | AGATTGTCAGCAATGCATCCTGC | CCTTCTTGATGTCATCATACTTGG |
eNos3 | CAGGACTGCACAGGAAATGTTC | AGCACATCAAAGCGGCCATTTC |
bFGF | CAACCGGTACCTTGCTATGAAG | CGTTTCAGTGCCACATACCAAC |
Primer sequences for genotype analysis | ||
Gsn+/+: 280 bp (Grohé et al., 2004) | ||
Gsn+1/+267 | GTGGAGCACCCCGAATT | CTCAGTTCAGGTATATCCATATCCATACAG |
Gsn−/−: 480 bp (Witke et al. 1995) | ||
Neo-3/-4 | ATTGAACAAGATGGATTGCAC | CGTCCAGATCATCCTGAT |