Table 1.
Bmax (fmol/mg protein) | KD (nm) | |
---|---|---|
WT, saline | 117.36 ± 7.72 | 1.09 ± 0.14 |
KO, saline | 112.77 ± 2.28 | 0.95 ± 0.10 |
WT, morphine | 108.95 ± 4.24 | 1.12 ± 0.17 |
KO, morphine | 119.77 ± 5.24 | 1.34 ± 0.17 |
The values (the mean ± SEM) based on the Scatchard plot analyses were obtained from 7–8 independent experiments. Bmax and KD were determined by fitting the data to a one-site binding model using nonlinear regression. The values of two-way ANOVA in the Bmax and KD values are F(1,26) = 2.02 and F(1,26) = 1.45, respectively.